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Immunofluorescence and immunohistochemistry Testis tissues or TM3 cells were fixed and processed for immunofluorescent or immunohistochemical staining as described previously [35]
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Although our data align with the notion that inhibiting NMDA receptor signalling in the hypothalamus decreases food intake and body weight 8,40 , earlier studies have demonstrated that NMDA receptor antagonism in the brainstem attenuates vagus-mediated meal-related satiety 6,7,42
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